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Conditions of optimal recombinant Human IL-5 Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
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Fax: 1-617-606-5019
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BP005006: Human IL-5 Protein with the N-His-Avi Tag
Source: The recombinant Human IL-5 Protein, (amino acids Ile20-Ser134) fused with a His-Avi tag at the N-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Human IL-5 protein with the His-Avi tag at the N-terminus has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human IL-5 protein with the N-His-Avi tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for the recombinant Human IL-5 R alpha protein with the N-hFc tag with the EC50 of 11.7 ng/ml determined by ELISA (QC Test). The affinity constant of 4.9 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human IL-5 Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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