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Conditions of optimal recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
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BP005465: Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein with the C-His-Avi Tag
Source: The recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein, (amino acids Phe42-Tyr995(ITGA5) acidic tail & Gln21-Asp728(ITGB1) basic tail) fused with a His-Avi tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer protein with the His-Avi tag at the C-terminus has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human ITGA5&ITGB1 protein with the C-His-Avi tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Anti-ITGA5&ITGB1 Antibody protein with the C-hFc tag with the EC50 of 16.2 ng/ml determined by ELISA.
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
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