Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form and multiple Fc backbone are available upon request.
BS050B:
Synthesis of the gene encoding the Fc fusion protein;
Molecular construction of the recombinant Fc fusion protein;
0.04 Liter transient production of the recombinant Fc fusion protein in CHO or HEK293 cells;
Protein A purification.BP001971-PRO-564: Human Liver Ferritin Protein
Source: Human Liver-derived.
Purity: > 96.0%.BP002537-ENZ-194: Recombinant Human FK506 Binding Protein 1B (FKBP1B)
Source: E. coli-derived.
Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 umole of suc-AAFP-pNA per minute at 25 in Tris-Hcl pH8.0 using chymotrypsin.
Purity: > 90.0% as determined by SDS-PAGE analysis.BP001503-C348: Recombinant Human Fibronectin Leucine Rich Transmembrane protein 1 (FLRT1) Protein
Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).BP001504-C349: Recombinant Human Fibronectin Leucine Rich Transmembrane protein 2 (FLRT2) Protein
Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 EU/ug).EK000083-EK0356: Human Fractalkine/CX3CL1 ELISA Kit
Range 156pg/ml-10,000pg/ml
Sensitivity < 50pg/ml
Specificity: Detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC < 0.2%.
Application: For quantitative detection of human Fractalkine in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Expiration: four months at 4°C and eight months at -20°C.BP000297-C461: Recombinant Human Fractalkine Protein
Source: HEK 293-derived.
Purity: > 95% by SEC-HPLC and reducing SDS-PAGE.
Endotoxin: < 0.1 ng/ug (1 IEU/ug).BP000297-CHM-005: Recombinant Rat Fractalkine Protein
Source: E. coli-derived.
Fully biologically active when compared to standard. Determined by its ability to chemoattract human monocytes using a concentration range of 5.0-10.0 ug/ml, corresponding to a specific activity of 100,000-200,000 units/mg.
Purity: > 97% by SDS-PAGE.BP000297-CHM-235: Recombinant Human Fractalkine Protein
Source: E. coli-derived.
The Biological activity is calculated by its ability to chemoattract human T-Lymphocytes using a concentration range of 5.0-10.0 ug/ml corresponding to a Specific Activity of 100,000-200,000 IU/mg.
Purity: > 97% by SDS-PAGE and RP-HPLC.BP000297-CHM-360: Recombinant Human Fractalkine Protein, His Tag
Source: E. coli-derived.
Purity: > 90% by SDS-PAGE.