Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
| Catalog No. | Product Name | Size | List Price (US$) | Quantity |
|---|
Conditions of optimal recombinant Mouse FLT3 Ligand Protein performance should be determined experimentally by the investigator.
Price/availability/specifications subject to change without notice. Unless otherwise indicated, our catalog and customized products are for research use only and not intended for human or animal diagnostic or therapeutic use.
Phone: 1-617-401-8149
Fax: 1-617-606-5019
Email: message@sydlabs.com
Or leave a message with a formal purchase
order (PO) Or credit card.
BP003497: Mouse FLT3 Ligand Protein with the C-His Tag
Source: The recombinant Mouse FLT3 Ligand Protein, (amino acids Gly27-Gln189) fused with a His tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight:The recombinant Mouse FLT3 Ligand protein with the His tag at the C-terminus has a predicted MW of 19.61 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Mouse FLT3 Ligand protein with the C-His tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Human FLT3 protein with the C-hFc tag with the EC50 of 39.3 ng/ml determined by ELISA (QC Test). The affinity constant of 0.18 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse FLT3 Ligand Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
BP004466: Mouse FLT3 Ligand Protein with the C-hFc Tag
Source: The recombinant Mouse FLT3 Ligand Protein, (amino acids Gly27-Gln189) fused with a hFc tag at the C-terminus, was produced in HEK 293 cells.
Molecular Weight: The recombinant Mouse FLT3 Ligand protein with the hFc tag at the C-terminus has a predicted MW of 45.3 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity: Immobilized recombinant Human FLT3 protein with the C-hFc tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for the recombinant Mouse FLT3 Ligand protein with the C-hFc tag with the EC50 of 16.7 ng/ml determined by ELISA (QC Test). The affinity constant of 13.81 nM as determined in SPR assay (Biacore T200).
Purity: >95% by SEC-HPLC and SDS-PAGE under reducing conditions.
Endotoxin Level: Less than 1 EU per ug of protein as determined by LAL method.
Formulation: Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution: It is recommended to reconstitute the lyophilized recombinant Mouse FLT3 Ligand Protein in sterile H2O to a protein concentration not less than 100 ug/ml. Centrifuge the tube before opening.
Copyright © 2009-2022 sydlabs.com. All rights reserved.
Use of this website means that you have read, understood, and accepted the Syd Labs Privacy Policy and Terms & Conditions.